6533b821fe1ef96bd127b963
RESEARCH PRODUCT
Additional file 7 of Intracellular osteopontin protects from autoimmunity-driven lymphoma development inhibiting TLR9-MYD88-STAT3 signaling
Celeste RizzelloValeria CancilaSabina SangalettiLaura BottiChiara RattiMatteo MilaniMatteo DugoFrancesco BertoniClaudio TripodoClaudia ChiodoniMario P. Colombosubject
description
Additional file 7: Supplementary Figure S4. Characterization of OPL239 and OPL241 DLBCL cell lines. A. Flow cytometry analysis showing the expression of B220, IgM, IgD and IgA in OPL239 and OPL241 cell lines. B. Hardy’s multiparametric flow cytometry panel illustrating the expression of CD93, CD21/35 and CD23 on OPL239 and OPL241 cell lines. C. Flow cytometry analysis showing the expression of TLR9 on OPL239 and OPL241 cell lines. D. RT-PCR analysis showing Spp1 mRNA level in overexpressing cell variants. E. Western blot for OPN protein expression (in presence or not of BFA, that blocks protein secretion) in parental and IRES-Green-based cell variants. 4T1 mammary cell line was used as positive control. F. Quantification of western blot analysis shown in figure 5D and F.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2023-01-01 |