6533b821fe1ef96bd127ba6c
RESEARCH PRODUCT
Characterization of the alcoholic fraction of vegetable oils by derivatization with diphenic anhydride followed by high-performance liquid chromatography with spectrophotometric and mass spectrometric detection
Guillermo Ramis-ramosJosé Vicente Gimeno-adelantadoErnesto F. Simó-alfonsoJosé Manuel Herrero-martínezMaría Jesús Lerma-garcíasubject
Resolution (mass spectrometry)Fraction (chemistry)Chemical FractionationSensitivity and SpecificityBiochemistryHigh-performance liquid chromatographyMass SpectrometryAnalytical ChemistryAcetic acidchemistry.chemical_compoundPlant Oilsmedia_common.cataloged_instanceEuropean unionDerivatizationChromatography High Pressure Liquidmedia_commonChromatographyEsterificationOrganic ChemistryExtraction (chemistry)Reproducibility of ResultsGeneral MedicineModels ChemicalchemistryAlcoholsMultivariate AnalysisDibenzoxepinsSaponificationdescription
Abstract Aliphatic and triterpene alcohols present in vegetable oils have been identified and determined by HPLC using UV–vis and MS detection after previous derivatization with diphenic anhydride. The alcoholic fraction was obtained by saponification, extraction and TLC (according to the European Union official procedure). Derivatization was performed in tetrahydrofuran in the presence of suspended grinded urea, which increases the reaction rate and yield. Derivatized extracts were chromatographed on a C8 column using gradient elution with acetonitrile/water mixtures containing 0.1% acetic acid, with UV–vis followed by negative-ion mode MS detection. Using linear discriminant analysis of the HPLC-MS data (extracted ion chromatograms), oil samples belonging to seven botanical origins (hazelnut, sunflower, corn, extra virgin olive, soybean, peanut and grapeseed) were correctly classified with excellent resolution among all the categories.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-08-07 | Journal of Chromatography A |