6533b821fe1ef96bd127c415

RESEARCH PRODUCT

Interaction of uridine with GABA binding sites in cerebellar membranes of the rat

Rosa GuarneriFederico PiccoliC. MocciaroP. Guarneri

subject

MaleCerebellumReceptors Cell SurfaceBiologyBinding CompetitiveBiochemistrygamma-Aminobutyric acidCellular and Molecular Neurosciencechemistry.chemical_compoundGABA receptorCerebellummedicineAnimalsBinding siteReceptorUridinegamma-Aminobutyric AcidGABAA receptorCell MembraneRats Inbred StrainsGeneral MedicineReceptors GABA-AUridineRatsmedicine.anatomical_structurenervous systemBiochemistryMechanism of actionchemistryAnticonvulsantsmedicine.symptommedicine.drug

description

The effect of uridine, a postulated anticonvulsant agent, on GABA receptors has been investigated. Uridine inhibits [3H]GABA binding to rat cerebellar buffer-washed membranes. Pretreatment of the membranes with Triton X-100 increases the effect of uridine on GABA-binding. The Scatchard analysis reveals that both high and low affinities of GABA for its receptors are affected by 1 mM uridine, while the apparent number of binding sites remains unchanged. The ability of uridine to interact competitively with GABA binding sites, also examined by the Lineweaver-Burk analysis, suggests a possible mechanism of action of this anticonvulsant agent, so including it among those compounds characterized by a GABAergic agonist activity.

yearjournalcountryeditionlanguage
1983-12-01Neurochemical Research
https://doi.org/10.1007/bf00964155