6533b821fe1ef96bd127c4f1

RESEARCH PRODUCT

Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

Antonio TiberiniRaffaele StassiSlavica MatićSalvatore DavinoSalvatore DavinoA. CarusoLivio TortaStefano PannoPatrizia Bella

subject

0106 biological sciences0301 basic medicineBst DNA polymeraseComputer scienceLoop-mediated isothermal amplificationSettore BIO/05 - ZoologiaviroidsPlant VirologyPlant ScienceReviewvirus01 natural sciences03 medical and health sciencesLAMPplant virologyprimersEcology Evolution Behavior and SystematicsEcologyviroid<i>Bst</i> DNA polymeraseBotanySettore AGR/12 - Patologia Vegetalereal-time monitoringeye diseasesDiagnosis methodsVisualizationprimer030104 developmental biologyQK1-989viruBiochemical engineeringloop-mediated isothermal amplification010606 plant biology & botany

description

In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.

yearjournalcountryeditionlanguage
2020-04-01Plants
10.3390/plants9040461https://www.mdpi.com/2223-7747/9/4/461