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RESEARCH PRODUCT
Role of calcium in E-selectin induced phenotype of T84 colon carcinoma cells
B BauderElise C. KohnG. AlaimoRiccardo AlessandroG. De LeoMonica D'amatoA. Flugysubject
BiophysicsAntineoplastic AgentsCD38BiochemistryCalcium in biologyCell MovementE-selectinTumor Cells CulturedHumansCalcium SignalingPhosphorylationCell adhesionMolecular BiologyCalcium signalingbiologyImidazolesCell BiologyTriazolesCalcium Channel BlockersRecombinant ProteinsCell biologyPhenotypeColonic NeoplasmsCancer cellbiology.proteinTyrosineCalciumNeural cell adhesion moleculeSignal transductionE-Selectindescription
The adhesion of cancer cells to the endothelium during the metastatic process involves the interaction of specific cell-cell adhesion receptors on the cell surface. E-selectin on endothelial cells and sialyl Lewis X carbohydrate component on tumor cells are mainly implicated in the adhesion of colon carcinoma cells to the endothelium of target organ. In this paper we show that binding of E-selectin to T84 colon tumor cells causes approximately a twofold increase in intracellular calcium concentration. In particular, using two inhibitors of receptor operated calcium channels, CAI and SK&F 96365, we present evidences that the augmentation in cytoplasmic calcium originates from ionic influx from extracellular sources. Furthermore, we demonstrated that modulation of [Ca2+]i by engagement of E-selectin receptor starts signal transduction pathways that affect cell spreading, tyrosine phosphorylation signaling, and cancer cell motility.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-02-01 | Biochemical and Biophysical Research Communications |