6533b822fe1ef96bd127cd67
RESEARCH PRODUCT
Isolation, purification and antibacterial effects of fusaproliferin produced by Fusariumsubglutinans in submerged culture.
Maria Adela ValeroJordi MañesJose SorianoGiuseppe MecaAlberto RitieniGiuseppe MecaIsabel Sospedrasubject
FusariumSpectrometry Mass Electrospray IonizationStaphylococcus aureusChromatographyMagnetic Resonance SpectroscopybiologyChemistryTerpenesGeneral MedicineNuclear magnetic resonance spectroscopyToxicologybiology.organism_classificationAntimicrobialHigh-performance liquid chromatographyAnti-Bacterial AgentsFusarium subglutinanschemistry.chemical_compoundFusariumProton NMREscherichia coliMycotoxinChromatography High Pressure LiquidFood ScienceAntibacterial agentdescription
To evaluate the fusaproliferin (FUS) production, Fusariumsubglutinans ITEM 2404 was grown in a liquid medium of potato being this mycotoxin purified by high-performance liquid chromatography (HPLC) with a C18 semipreparative column using a mobile phase of acetonitrile/H(2)O using gradient conditions. The purity of the fusaproliferin was verified by analytical HPLC, ultraviolet absorbance measurements, LC/MS-MS, (1)H NMR spectroscopy. The isolated FUS was shown to be free of impurities and can be used as a standard for routine analysis. The pure fusaproliferin was utilized to study the biological activity on Escherichiacoli and Staphylococcusaureus. This study demostred that FUS not showed significant antimicrobial activity against these microorganisms.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-10-01 | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association |