Protection of rabbits against rabbit viral haemorrhagic disease with a vaccinia-RHDV recombinant virus

6533b822fe1ef96bd127cd74

RESEARCH PRODUCT

Protection of rabbits against rabbit viral haemorrhagic disease with a vaccinia-RHDV recombinant virus

S. Laurent Jacqueline Gelfi E. Boilletot F. Petit Stéphane Bertagnoli C. Boucraut-baralon J. Chantal G Le Gall Jean-françois Vautherot Denis Rasschaert

subject

Injections Intradermal Hemorrhagic Disease Virus Rabbit viruses [SDV]Life Sciences [q-bio]Administration Oral Vaccinia virus Genome Viral Biology Antibodies Viral Recombinant virus Virus Cell Line law.invention 03 medical and health sciences chemistry.chemical_compound law Animals Poxviridae Orthopoxvirus ComputingMilieux_MISCELLANEOUS Caliciviridae Infections 030304 developmental biology Viral Structural Proteins Vaccines Synthetic 0303 health sciences General Veterinary General Immunology and Microbiology 030306 microbiology Public Health Environmental and Occupational Health Viral Vaccines biology.organism_classification Virology Caliciviridae 3. Good health [SDV] Life Sciences [q-bio]Infectious Diseases chemistry Capsid Recombinant DNA Molecular Medicine VACCINATION Rabbits Vaccinia

description

In order to protect domestic and wild rabbits against RVHD, we constructed a recombinant vaccinia-RHDV virus, using the Copenhagen strain of the vaccinia virus. This recombinant virus expressed the RHDV capsid protein (VP60). Analysis of the expressed product showed that the recombinant protein, which is 60 kDa in size, was antigenic as revealed by its reactions in immunoprecipitation and indirect immunofluorescence with the antibodies raised against RHDV. The recombinant virus induced high level of RHDV specific antibodies in rabbits following immunization. Inoculations by both the intradermal and oral routes allow protection of animals against a challenge with virulent RHDV.

year	journal	country	edition	language
1996-04-01

https://hal.inrae.fr/hal-02697434

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