6533b822fe1ef96bd127ce49

RESEARCH PRODUCT

In vivo anti-inflammatory activity of saponins from Bupleurum rotundifolium

Salvador MáñezJosé-luis RíosM. Carmen RecioRosa M. GinerPilar González NavarroMiguel Cerdá-nicolás

subject

chemistry.chemical_classificationPlants MedicinalTraditional medicineChemistrymedicine.drug_classAnti-Inflammatory AgentsSaponinGeneral MedicineSaponinsGeneral Biochemistry Genetics and Molecular BiologyAnti-inflammatoryMiceTriterpeneIn vivoBupleurum rotundifoliummedicineAnimalsTetradecanoylphorbol AcetateFemaleGeneral Pharmacology Toxicology and PharmaceuticsEchinocystic acidSpectral dataEar edema

description

Seven oleanane-type triterpene saponins were isolated from the methanolic extract of the aerial parts of Bupleurum rotundifolium. They were identified on the basis of their spectral data as 3-O-[alpha-L-rhamnopyranosyl (1--2)-beta-D-glucopyranosyl (1--2)-beta-D-glucopyranosyl]-28-O-[beta-D-glucopyranosyl (1--2)-beta-D-glucopyranosyl] echinocystic acid (saponin 1), 3-O-[alpha-L-rhamnopyranosyl (1--2)-beta-D-glucopyranosyl (1--2)-beta-D-fucopyranosyl] 11-methoxy-primulagenin A (saponin 2), rotundioside E (saponin 3), rotundioside F (saponin 4), 3beta-sulfate, 28-O-[beta-D-glucopyranosyl (1--6)-beta-D-glucopyranosyl (1--2)-beta-D-glucopyranosyl (1--2)-beta-D-glucopyranosyl] ester of primulagenin A (saponin 5), rotundioside C (saponin 6) and 3-O-[alpha-L-rhamnopyranosyl (1--2)-beta-D-glucopyranosyl (1--2)-beta-D-fucopyranosyl] 11-methoxy-16beta,21alpha,28-trihydroxyolean-12-ene (saponin 7). All these saponins proved to be effective against TPA-induced ear edema in mice. Their ID50 were determined to be 248, 288, 128, 99 and 297 nmol/ear for saponin 1, 2, 3, 4 and 6, respectively. Saponins 3 and 6 were also active on a TPA multiple-dose model of skin chronic inflammation.

yearjournalcountryeditionlanguage
2001-03-03Life Sciences
https://doi.org/10.1016/s0024-3205(00)01019-5