Ādas mezenhimālo cilmes šūnu fenotipa raksturojums pēc neirotrofo faktoru inducētas neirodiferenciācijas

6533b822fe1ef96bd127d12d

RESEARCH PRODUCT

Ādas mezenhimālo cilmes šūnu fenotipa raksturojums pēc neirotrofo faktoru inducētas neirodiferenciācijas

subject

BDNF ReNcell CX Sigma-1 receptors neiroektoderma Bioloģija mezenhimālās cilmes šūnas

description

Ādas mezenhimālās cilmes šūnas (Ā-MCš), pakļautas specifiskiem faktoriem, spēj diferencēties par neironiem un glijas šūnām. Maģistra darba mērķis bija analizēt Ā-MCš fenotipu pēc neirotrofo faktoru inducētas neirodiferenciācijas. Neiroektodermas gēnu ekspresiju Ā-MCš analizēja ar qPCR, cilmes šūnu pluripotences un mezenhimālo marķieru ekspresiju ar plūsmas citometriju, Sigma-1 receptora ekspresiju (SigmaR1) ar qPCR un IF, kā arī BDNF sekrēciju ar ELISA. Ā-MCš fenotipu salīdzināja ar ReNcell CX šūnām. Retīnskābes indukcijas barotnē novēroja nervu kores gēnu ekspresijas pieaugumu. Cilmes šūnu marķieru ekspresija pēc diferenciācijas kritās. Mezenhimālo marķieru ekspresija samazinājās vienā no testētajām donoru šūnu līnijām. SigmaR1 visaugstāk ekspresēja nediferencētas Ā-MCš. Švāna šūnu barotnē šūnas pastiprināti sekretēja BDNF.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/28556

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