6533b822fe1ef96bd127d818
RESEARCH PRODUCT
Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA.
José P. MartínezM L GilManuel Casanovasubject
Fungal proteinMembrane GlycoproteinsHyphaDNA synthesisProtoplastsGerm tubeGeneral MedicineBiologybiology.organism_classificationBiochemistryMicrobiologyCell wallFungal ProteinsMolecular Weightchemistry.chemical_compoundBiochemistrychemistryCell WallCandida albicansGeneticsSecretionGrowth inhibitionCandida albicansMolecular BiologyEdetic Aciddescription
Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37 degrees C in EDTA-free medium. High-molecular-weight mannoproteins (HMWM) that are mycelium-specific wall components, and particularly a 260-kDa species (HMWM-260), were absent in the wall of cells grown under germination conditions in the presence of EDTA. Synthesis of the HMWM-260 species was not inhibited but its incorporation (secretion) into the wall structure seemed to be blocked in EDTA-treated cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-06-01 | Archives of microbiology |