Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii)

6533b822fe1ef96bd127d8b4

RESEARCH PRODUCT

Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii)

J.g. Herranz-jusdado Carina Caldeira Borys Dzyuba Martin Pšenička Patrick H. Martin Carles Soler Anthony Valverde Sandra Hernández-ibáñez Juan F. Asturiano Victor Gallego

subject

ZOOLOGIA Sperm quality Zoology Aquatic Science PRODUCCION ANIMAL 03 medical and health sciences 0302 clinical medicine Sturgeon Aquaculture 14. Life underwater Salmo Spermatozoa track Sperm motility 030304 developmental biology 0303 health sciences Fish migration 030219 obstetrics & reproductive medicine Diadromous fish species biology business.industry Frame rate Aquatic animal Acipenser baerii biology.organism_classification Sperm business Counting chamber Magnification lens

description

[EN] It is essential to define an optimized standard method to assess the fish sperm quality to minimize the differences between the results obtained by different laboratories. Only this optimization and standardization can make them useful from academia to industry. This study presents the validation of sperm motility assessment using a CASA-Mot system for three endangered diadromous fish species: European eel (Anguilla anguilla), Atlantic salmon (Salmo salar) and Siberian sturgeon (Acipenser baerii). To attain this goal, different technical and data processing methods were tested: 1) magnification lens (×10 and ×20), 2) Spermtrack® reusable chambers (10 and 20¿¿m depth) and 3) different frame rates (50¿¿¿FR¿¿¿250). The results suggested that the sperm motility assessment for eel, salmon and sturgeon should be performed at 200, 250 and 225¿frames¿s¿1, respectively. Moreover, to obtain a high number of analysed spermatozoa in less time and a natural movement of the sperm cells, it is recommended to use ×10 objective and 20¿¿m depth. In conclusion, different technical settings influence sperm kinetic parameters and should be validated for each fish species to allow the comparison of results between laboratories.

year	journal	country	edition	language
2019-03-01

10.1016/j.aquaculture.2018.12.001 https://doi.org/10.1016/j.aquaculture.2018.12.001