6533b823fe1ef96bd127e358

RESEARCH PRODUCT

Immunohistochemical localization of the pro-peptide processing enzymes PC1/PC3 and PC2 in the human anal canal.

Nabil G. SeidahEberhard WeiheEberhard WeiheRobert DayDieter HörschMartin K.-h. SchäferMartin K.-h. Schäfer

subject

endocrine systemPathologymedicine.medical_specialtyPhysiologyProhormoneNeuropeptideRectumAnal CanalBiologyBiochemistryImmunoenzyme TechniquesCellular and Molecular NeuroscienceEndocrinologymedicineChromograninsAspartic Acid EndopeptidasesHumansSubtilisinsAnal Transitional ZoneNeuronsNeuropeptidesAnal canalNeurosecretory SystemsEpitheliumNeoplasm Proteinsmedicine.anatomical_structureProprotein Convertase 2Fluorescent Antibody Technique DirectChromogranin AProprotein ConvertasesStem cellImmunostainingmedicine.drug

description

Abstract HORsch, D., R. Day, N. G. Seidah, E. Weihe and M. K.-H. SchAFer. Immunohistochemical localization of the pro-peptide processing enzymes PC1/PC3 and PC2 in the human anal canal. Peptides 18(5) 755–760, 1997.—The distribution of prohormone/pro-peptide convertases PC1/PC3 and PC2 was investigated in the human anal canal by immunohistochemistry. Both prohormone convertases exhibited region-specific distribution patterns and were observed in neural and neuroendocrine cells and in nonneuroendocrine cellular elements. PC1/PC3 immunoreactivity was present in enteric neurons, subsets of nerve fibers, and neuroendocrine cells, and also in epithelial cells like intestinal stem cells, and a subpopulation of squamous cells. Enteric neurons were PC2 immunoreactive, whereas PC2 immunostaining in nerve fibers was slightly above background levels. Few neuroendocrine cells contained PC2 immunoreactivity, which were located predominantly in the anal transitional zone. In the squamous epithelium, the basal cell layer stained for PC2. The tissue-specific distribution of PC1/PC3 and PC2 indicates region-specific processing of peptides with regulatory functions in the anal canal and further supports the hypothesis that neuropeptides are important regulators of anal functions.

yearjournalcountryeditionlanguage
1997-01-01Peptides
10.1016/s0196-9781(96)00029-0https://pubmed.ncbi.nlm.nih.gov/9213372