6533b823fe1ef96bd127e3b4
RESEARCH PRODUCT
In Vitro Analysis of the Two-Component System MtrB-MtrA from Corynebacterium glutamicum▿ †
Susanne MorbachGottfried UndenReinhard KrämerJens KrämerNina Mökersubject
PhosphataseCorynebacteriumEnzyme ActivatorsMicrobiologyCorynebacterium glutamicumEnzyme activatorBacterial ProteinsOsmotic PressurePhosphorylationMolecular BiologyHistidinebiologyAutophosphorylationRNA-Binding ProteinsCations Monovalentbiology.organism_classificationAdaptation PhysiologicalTwo-component regulatory systemPhosphoric Monoester HydrolasesCorynebacterium glutamicumBiochemistryLiposomesPhosphorylationATP-Binding Cassette TransportersSignal TransductionTranscription Factorsdescription
ABSTRACT The two-component system MtrBA is involved in the osmostress response of Corynebacterium glutamicum . MtrB was reconstituted in a functionally active form in liposomes and showed autophosphorylation and phosphatase activity. In proteoliposomes, MtrB activity was stimulated by monovalent cations used by many osmosensors for the detection of hypertonicity. Although MtrB was activated by monovalent cations, they lead in vitro to a general stabilization of histidine kinases and do not represent the stimulus for MtrB to sense hyperosmotic stress.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2007-05-01 |