6533b823fe1ef96bd127e648

RESEARCH PRODUCT

Time-resolved X-ray scattering as a tool to probe heme proteins structural dynamics in solution

Matteo LevantinoAntonio CupaneM CammarataB ValloneM Brunori

subject

Dynamic Structural ScienceSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)

description

Time-resolved wide-angle X-ray scattering (TR-WAXS) is a recently developed technique allowing to probe global structural changes of proteins in solution. We have used TR-WAXS to investigate large conformational changes of heme proteins (wild-type and mutant hemoglobin, neuroglobin, etc.) that cannot take place when these macromolecules are in a crystalline environment. Our data revealed detailed information on kinetic and thermodynamic properties of the investigated proteins and demonstrate the potentiality of the TR-WAXS technique.

yearjournalcountryeditionlanguage
2013-01-01
http://hdl.handle.net/10447/80304