Orthogonal electrophoretic fractionation of DNA in agarose gels.

6533b823fe1ef96bd127eb6e

RESEARCH PRODUCT

Orthogonal electrophoretic fractionation of DNA in agarose gels.

Rainer Barbieri Daniele Bellavia Giorgia Sisino Giada Sisino

subject

Gel electrophoresis Electrophoresis Agar Gel Chromatography Gel electrophoresis of nucleic acids Cell Biology Fractionation DNA Biology Molecular biology Interleukin-10 chemistry.chemical_compound Electrophoresis chemistry Agarose RNA Messenger Molecular Biology DNA Temperature gradient gel electrophoresis Southern blot

description

We developed an electrophoretic procedure, using Voltage Gradient Gel Electrophoresis (VGGE), which allows to obtain both an improvement of the resolution power of the system in orthogonal fractionation of DNA and, mainly, an about fourfold enhancement of hybridization signals in Southern blotting applications.

year	journal	country	edition	language
2008-08-01	Molecular and cellular probes

10.1016/j.mcp.2008.05.002 https://pubmed.ncbi.nlm.nih.gov/18586454

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