6533b823fe1ef96bd127ebba

RESEARCH PRODUCT

Immune suppression in advanced chronic fascioliasis: an experimental study in a rat model.

Carmen PunzónManuel PresnoMaria A. García-bodelónNúria GironènesIsabel Chico-caleroM. Adela ValeroSantiago Mas-coma

subject

FascioliasisMononuclear cell proliferationmedicine.medical_treatmentT cellT-LymphocytesImmune systemmedicineImmunology and AllergyAnimalsLymphocyte CountB cellImmunosuppression TherapyB-LymphocytesbiologyIsotypeAntigens DifferentiationFasciolaBlood Cell CountRatsChronic infectionDisease Models AnimalInfectious DiseasesCytokinemedicine.anatomical_structureImmunoglobulin GImmunologybiology.proteinCytokinesEgyptAntibodyCell DivisionSpleen

description

Chronicity and Th2 immune responses are features of helminth infections in humans. The liver fluke promotes its own survival through several strategies to down-regulate the immune response of the host during the early phase of infection. However, there is no evidence that this modulation occurs much later. The immune response in advanced chronic fascioliasis was analyzed in an experimental rat model at 20 weeks after infection. Cytokine quantification in infected rat serum revealed basal levels. The predominant immunoglobulin (Ig) isotype was IgG1. Flow cytometry analysis of T cell (CD3 + , CD4 + , and CD8a + ), B cell (CD45R + ), and macrophage (CD11b + ) populations in spleens showed no significant differences between infected and control rats. Mononuclear cell proliferation in the spleen in response to T and B mitogens was strongly inhibited in infected versus control rats. During early chronic infection, there is a predominance of a Th2 response, which decreases in advanced chronic infection characterized by a persistent immune suppression.

yearjournalcountryeditionlanguage
2006-09-13The Journal of infectious diseases
10.1086/514822https://pubmed.ncbi.nlm.nih.gov/17436231