6533b823fe1ef96bd127ed5f
RESEARCH PRODUCT
Hookworm-like eggs in children’s faecal samples from a rural area of Rwanda
Fernando J. Bornay-llinaresMaría José Irisarri-gutiérrezRafael ToledoCarla Muñoz-antoliL. AcostaLucy Anne ParkerJosé Guillermo Estebansubject
AncylostomatoideaRural PopulationVeterinary medicineHookworm eggs Kato-Katz method misclassification RwandaANCYLOSTOMATOIDEAAdolescent030231 tropical medicineBiologyPolymerase Chain ReactionSensitivity and Specificity03 medical and health sciencesFecesHookworm InfectionsSoilfluids and secretions0302 clinical medicineparasitic diseasesParasite Egg CountAnimalsHumansChildParasite Egg CountFecesRwandaGeneral MedicineArticlesCross-Sectional StudiesHookworm InfectionsKato katz030211 gastroenterology & hepatologyhuman activitiesRural populationSequence Analysisdescription
Background: Hookworm eggs identification and quantification is usually carried out by Kato-Katz method. However various structures present in the smear may be confused with eggs of such parasites. Objective: To document the presence of structures in Kato-Katz slides that could initially be misinterpreted as hookworm eggs. Method: 497 faecal samples were analysed by Kato-Katz technique, diphasic concentration technique, agar-plate coprocultive and larvae obtained were analysed by PCR and characterized by sequencing. Result: Hookworm-like eggs were found in 159 (32%) of the samples by Kato Katz, finally identified as Caenorhabditis elegans by PCR technique. Conclusion: The diagnosis of human hookworm eggs, only by the use of Kato Katz technique can lead to false positives because of similarities with eggs of other free-living worms, from wet soils like those of Rwanda that could contaminate stool samples. Keywords: Hookworm eggs, Kato-Katz method, misclassification, Rwanda
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2016-05-09 | African Health Sciences |