6533b823fe1ef96bd127eda8
RESEARCH PRODUCT
Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections.
Dolores BernalRafael ToledoAna EspertJosé Enrique De La RubiaJosé Guillermo EstebanAntonio MarcillaInés Carpenasubject
ProteomicsMolecular Sequence DataBiologyProteomicsBiochemistrySpecies SpecificityHeat shock proteinCricetinaeEchinostomaAnimalsAmino Acid SequenceRats WistarCytoskeletonMolecular BiologyPeptide sequenceEchinostomiasisMesocricetusAldolase AProteinsTropomyosinHsp70RatsDisease Models AnimalSecretory proteinBiochemistryAcute DiseaseChronic Diseasebiology.proteindescription
In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyosin, and paramyosin; glycolytic enzymes like enolase, glyceraldehyde 3P dehydrogenase, and aldolase; detoxifying enzymes like GSTs; and stress proteins like heat shock protein (Hsp) 70. Among these proteins, both actin and, to a lesser extent, Hsp70, exhibited differential expression patterns between chronic and acute infections in the Echinostoma-rodent model, suggesting that these proteins may play a role in the survival within the host.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-03-31 | Proteomics |