Bromoviridae dzimtas augu vīrusa nanodaļiņu konstruēšana un raksturošana

6533b823fe1ef96bd127efd6

RESEARCH PRODUCT

Bromoviridae dzimtas augu vīrusa nanodaļiņu konstruēšana un raksturošana

subject

tetanusa toksīns VLP Bioloģija lopbarības pupiņas raibumu vīruss apvalka proteīns

description

Bakalaura darba mērķis bija iegūt VLP E.coli no pilna un par 24 aa N-gala saīsināta CCMV CP VLP un balstoties uz šīm konstrukcijām, izveidot CP konstrukcijas, kas N- vai C- galā satur TT830 peptīda sekvenci. Bakalaura darbs izstrādāts no 2015. gada septembra līdz 2016. gada maijam Latvijas Biomedicīnas pētījumu un studiju centrā, Augu virusoloģijas laboratorijā Dr. biol. Andra Zeltiņa un Dr. biol. Inas Baļķes vadībā. Pētījumu objekts ir lopbarības pupiņas raibumu vīruss - CCMV. Bakalaura darba laikā tika izveidotas piecas CCMV CP ekspresijas konstrukcijas - pET42-CCMV, pET42-CCMV–Ntt830, pET42-CCMV–Ctt830, pET42-Δ24CCMV, pET42-Δ24CCMV-Ntt830 un pET42-Δ24CCMV–Ctt830. Ekspresijas pārbaudes analīze uzrādīja, ka visi CP varianti ekspresējas E.coli un ir šķīstoši, kā arī veido VLP. CCMV, Δ24CCMV, CCMV-Ntt830 un Δ24CCMV-Ntt830 veido T=3 simetrijas daļiņas, bet CCMV-Ctt830 un Δ24CCMV-Ctt830 veido bacilformas vai pleomorfas daļiņas. CCMV, Δ24CCMV, CCMV-Ntt830 un Δ24CCMV-Ntt830 var izmantot ķīmiskai epitopu pievienošanai. Atslēgas vārdi: lopbarības pupiņas raibumu vīruss, apvalka proteīns, tetanusa toksīns, VLP

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/33180

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
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- PE1Mathematics
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