6533b823fe1ef96bd127f5cb
RESEARCH PRODUCT
Chondroitin AC lyase activity is related to virulence of fish pathogenic Flavobacterium columnare.
Marja TiirolaL.-r. SuomalainenE. T. Valtonensubject
Veterinary (miscellaneous)VirulenceAquatic ScienceDegradative enzymeFlavobacteriumBacterial AdhesionMicrobiologychemistry.chemical_compoundFish DiseasesPlasmidFlavobacteriaceae InfectionsChondroitinAnimalsFinlandbiologyChondroitin LyasesVirulencebiology.organism_classificationLyaseMolecular biologyIn vitrochemistryOncorhynchus mykissFlavobacterium columnarebiology.proteinChondroitin AC lyasePlasmidsdescription
The virulence of eight Flavobacterium columnare strains was studied to find correlations between several virulence-related factors and virulence. Virulence was tested in vivo using rainbow trout, Oncorhynchus mykiss (Walbaum). Suggested virulence-related factors such as production of the degradative enzyme chondroitin lyase, plasmid occurrence and adhesion capability were studied in vitro. Infection with the four most virulent strains resulted in 95-100% mortality within 114 h. Chondroitin lyase activity was found to be significantly related to the virulence of the strains at 25 degrees C and it was also shown to be temperature-dependent, being higher at 25 degrees C than at 20 degrees C. Virulence was not plasmid associated. The adhesion capability of the strains in vitro varied substantially when tested on crude mucus-coated slides and no statistical relationship between adhesion and virulence was found using this method.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-12-16 | Journal of fish diseases |