6533b824fe1ef96bd127ffd2
RESEARCH PRODUCT
Novel group A rotavirus G8 P[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs
Joaquim SegalésI. Galindo-cardielM. Fernández-jiménezJuan José BadiolaE. FantovaLluís LujánJorge BlancoJavier BuesaJ. Espadasubject
DiarrheaRotavirusGenotypevirusesSheep DiseasesWeaningBiologymedicine.disease_causePolymerase Chain ReactionMicrobiologyGroup AChromatography AffinityRotavirus InfectionsDisease OutbreaksFeces03 medical and health sciencesOvine rotavirusfluids and secretionsRotavirusmedicineAnimalsOvine diarrheic syndromeGenotypingSheep DomesticFeces030304 developmental biology0303 health sciences[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal HealthSheepGeneral VeterinarySequence Analysis RNA030306 microbiologyOutbreakGeneral MedicineImmunohistochemistryVirology3. Good healthweaned lambsDiarrheaSpainRNA Viralrotavirus group Amedicine.symptomdescription
International audience; Rotavirus is a worldwide major cause of diarrhea outbreaks in neonatal ruminants. An outbreak of ovine diarrheic syndrome (ODS) in 50-75 days-old lambs (weaned lambs) is described. Fecal immunochromatography and intestinal immunohistochemistry for rotavirus group A were performed. In addition, semi-nested multiplex RT-PCR for G and P rotavirus genotyping in combination with sequencing were performed, to support the diagnosis and identify the viral strain. A novel ovine rotavirus group A G8 P[1] strain was determined as the main cause of the ODS observed, whereas other pathogens were ruled out.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-03-29 | Veterinary Microbiology |