6533b824fe1ef96bd1280601
RESEARCH PRODUCT
Combination of multiplex PCR and PCR-DGGE for monitoring common sourdough-associated Lactobacillus species
Luca SettanniS ValmorriD Van SinderenG SuzziA PaparellaA. Corsettisubject
PCR multiplex DGGE impasti acididescription
A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-01-01 |