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RESEARCH PRODUCT
Direct vapor generation Fourier transform infrared spectrometric determination of ethanol in blood
Salvador GarriguesMiguel De La GuardiaAmparo Pérez-poncesubject
Detection limitChromatographyInfraredChemistryCoefficient of variationDrop (liquid)Analytical chemistryInfrared spectroscopyPlasmaBiochemistryAnalytical ChemistryAbsorbancesymbols.namesakeFourier transformsymbolsEnvironmental ChemistrySpectroscopydescription
Abstract A new procedure is proposed for a direct determination of ethanol in plasma and whole blood. The method is based on the injection of a discrete sample volume of 10 μl into an electrically heated Pyrex glass reactor in which, at a temperature of 90 °C, the ethanol is volatilized and introduced by means of a N 2 carrier flow inside a long-path infrared gas cell and the corresponding flow analysis recording registered as a function of time. The measurement of the area of the flow injection recording, obtained from the absorbance of the transient signal in the range 1150−950 cm −1 , allows the direct quantification of ethanol upto 2 g l −1 , with a limit of detection of 0.020 g l −1 and coefficient of variation between 0.3 and 1.9% for three replicate analyses of the same sample. The analysis frequency of the method is 40 h −1 , and it can be applied to a single drop of finger blood.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-12-01 | Analytica Chimica Acta |