6533b824fe1ef96bd1280ac2
RESEARCH PRODUCT
A DNA region ofTorulaspora delbrueckii containing theHIS3 gene: sequence, gene order and evolution
Jose Antonio PrietoEladio BarrioFrancisca Randez-gilElena Aller-arranzsubject
Saccharomyces cerevisiae ProteinsTranscription GeneticSequence analysisMolecular Sequence DataSaccharomyces cerevisiaeCell Cycle ProteinsBioengineeringBiologyApplied Microbiology and BiotechnologyBiochemistryHomology (biology)DEAD-box RNA HelicasesEvolution MolecularFungal ProteinsOpen Reading FramesTorulaspora delbrueckiiGeneticsAmino Acid SequenceCloning MolecularORFSDNA FungalGeneHydro-LyasesPhylogenyGeneticsBase SequenceMethyltransferasesbiology.organism_classificationMolecular biologygenomic DNASaccharomycetalesChromosomal regionSequence AlignmentRNA HelicasesBiotechnologydescription
We cloned a genomic DNA fragment of the yeast Torulaspora delbrueckii by complementation of a Saccharomyces cerevisiae his3 mutant strain. DNA sequence analysis revealed that the fragment contained two complete ORFs, which share a high similarity with S. cerevisiae His3p and Mrp51p, respectively. The cloned TdHIS3 gene fully complemented the his3 mutation of S. cerevisiae, confirming that it encodes for the imidazoleglycerol-phosphate dehydrate of T. delbrueckii. Two additional ORFs, with a high homology to S. cerevisiae PET56 and DED1 genes, were mapped upstream and downstream from TdHIS3 and TdMRP51, respectively. This genetic organization is analogous to that previously found in Saccharomyces kluyveri and Zygosaccharomyces rouxii. The evolutionary significance of gene order in this chromosomal region is analysed and discussed. The GeneBank Accession No. for the sequenced DNA fragment is AY238990. Copyright © 2003 John Wiley & Sons, Ltd.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-12-10 | Yeast |