Pulsējošā elektromagnētiskā un gaismas starojuma ietekme uz baktēriju dzīvotspēju

6533b824fe1ef96bd1280e74

RESEARCH PRODUCT

Pulsējošā elektromagnētiskā un gaismas starojuma ietekme uz baktēriju dzīvotspēju

subject

nāvējošās oscilācijas R.R.Raifs S.aureus E.coli fluorescence Bioloģija

description

Baktēriju augšanu ir iespējams ietekmēt ar elektromagnētisko starojumu. Šī darba mērķis bija pētīt Escherichia coli un Staphylococus aureus kultūru dzīvotspēju pulsējošā elektromagnētiskā un gaismas starojuma ietekmē, vadoties pēc R. R. Raifa izstrādātās mikroorganismu nāvējošās oscilācijas hipotēzes. Šim nolūkam tika replicēta R.R. Raifa elektromagnētiskā starojuma ierīce, kas apstaroja kultūras režīmos no 600 Hz līdz 1000 Hz laika periodā 10 sekundes. Dzīvotspēja tika noteikta, mikroskopējot un skaitot šūnas, kas tika iekrāsotas ar ThermoFisher LIVE/DEAD BacLight Bacterial Viability Kit. Tika noteiktas divas atšķirīgas baktericīdas frekvences – 800 Hz (E.coli) un 727 Hz (S.aureus), ar kurām tika nonāvēti 34,32% E.coli un 71,69% S.aureus šūnu.

year	journal	country	edition	language
2020-01-01

https://dspace.lu.lv/dspace/handle/7/50693

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