6533b824fe1ef96bd12813e3
RESEARCH PRODUCT
Investigation of the dynamics of bacteriorhodopsin
J. HeidemeierH. FormanekFritz G. ParakB. HessM. EngelhardM. FischerK. D. Kohlsubject
inorganic chemicalsNuclear and High Energy PhysicsbiologyChemistryBacteriorhodopsinAtmospheric temperature rangeCondensed Matter PhysicsAtomic and Molecular Physics and OpticsIonCrystallographyMembraneOxygen atomLipid contentbiology.proteinMossbauer spectraPhysical and Theoretical ChemistryLipid bilayerdescription
Bacteriorhodopsin (bR) converted to the blue form by deionization has been reconstituted to the active purple membrane by addition of57Fe ions. Mossbauer spectra measured in a wide temperature range reveal Fe3+ binding places with oxygen atoms in the neighbourhood. No evidence for a well defined functional binding place of the iron has been found. On a timescale faster 100 ns the purple membrane shows increasing flexibility above 200 K. In order to analyse the influence of the lipids, a bacteriorhodopsin sample where the lipid content has been increased artificially by the incorporation of DMPC as well as a sample consisting of lipid bilayer have been investigated.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1990-07-01 | Hyperfine Interactions |