Divdimensionālās šķidruma hromatogrāfijas augstas izšķirtspējas masspektrometrijas metodes izstrāde mikotoksīnu noteikšanai Puer tējās

6533b824fe1ef96bd1281848

RESEARCH PRODUCT

Divdimensionālās šķidruma hromatogrāfijas augstas izšķirtspējas masspektrometrijas metodes izstrāde mikotoksīnu noteikšanai Puer tējās

Estefanija Bogdanova

subject

HEART-CUTTING-2D-LC MIKOTOKSĪNI TOF AUGSTAS IZŠĶIRTSPĒJAS MASSPEKTROMETRIJA DIVDIMENSIONĀLĀ ŠĶIDRUMA HROMATOGRĀFIJA Ķīmija

description

Maģistra darba ietvaros tika izstrādāta inovatīva divdimensionālās šķidruma hromatogrāfijas metode tandēmā ar augstas izšķirtspējas nolidojuma laika masspektrometriju (2D-LC-TOF-HRMS) 70 “jauno” un reglamentēto mikotoksīnu noteikšanai Puer tēju matricā. Šis uzdevums tika izvirzīts ar mērķi palielināt nosakāmo mikotoksīnu skaitu, uzlabot metodes selektivitāti un novērst traucējošo matricas komponentu efektu, pielietojot savienojumu atdalīšanu ar 2D-LC sistēmas palīdzību. Metode tika validēta saskaņā ar Eiropas Savienības analīzes metodes vadlīnijām. Izstrādātā online-heart-cutting-2D-HPLC-HRMS metode tika pielietota 20 Latvijā komerciāli pieejamajiem Puer tēju paraugiem, veicot analizēto mikotoksīnu sastopamības apkopojumu un rezultātu izvērtējumu. Literatūras apskatā ir apkopota informācija par mikotoksīnu īpašībām, to izdalīšanu no pārtikas produktiem, paraugu sagatavošanas un noteikšanas metodēm, kā arī divdimensionālās šķidruma hromatogrāfijas veidiem un darbības principiem.

year	journal	country	edition	language
2019-01-01

https://dspace.lu.lv/dspace/handle/7/46749

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