Penicillium lanoso - viride glikoproteīnu preparātu fermentatīvo aktivitāšu raksturojums

6533b825fe1ef96bd12822b6

RESEARCH PRODUCT

Penicillium lanoso - viride glikoproteīnu preparātu fermentatīvo aktivitāšu raksturojums

Diāna Marcinkēviča

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Darba mērķis bija izpētīt, kādas enzimātiskās aktivitātes piemīt glikoproteīna preparātam, kas izdalīts no Penicillium lanoso-viride mikroskopiskajām sēnēm. Lai sasniegtu mērķi, Escherichia coli celma DH5α kompetentās šūnas tika transformētas ar pUC18 plazmīdu DNS, kultivētas un no tām tika izdalīts superspiralizēts (ccc) plazmīdu DNS. Tika noteiks, kā proteīna preparāts iedarbojas uz superspiralizētu plazmīdu DNS dažādu faktoru ietekmē (EDTA, temperatūra un cinka joni), kā arī uz relaksētu un lineāru plazmīdu DNS. Noskaidrojās, ka glikoproteīna preparātam ir dažādas enzimātiskās aktivitātes – AMP dezamināzes un endonukleāzes, tādēļ tika veikta frakcionēšana uz DEAE celulozes, lai atdalītu proteīnus ar dažādām aktivitātēm. Tika noteiktas endonukleāzes, AMP dezamināzes un fosfatāzes aktivitātes iegūtajās frakcijās, kā arī veikta proteīna preparāta elektroforēze SDS-PAA gēlā. Atslēgvārdi: AMP dezamināze, superspiralizēts plazmīdu DNS, endonukleāze.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/18939

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