6533b825fe1ef96bd12823e0
RESEARCH PRODUCT
On the interaction of doxorubicin with polynucleotides
Marta AiroldiGiampaolo BaroneGiuseppe GennaroAnna Maria Giuliani RicciM. Giustinisubject
DoxorubicinPolynucleotideSettore CHIM/03 - Chimica Generale E Inorganicamolecular mechanics calculationsfluorescencecircular dichroismdescription
The interaction of doxorubicin (DX) with model polynucleotides poly(dG-dC)·poly(dG-dC) (polyGC), poly(dA-dT)·poly(dA-dT) (poly-AT), and calf thymus DNA has been studied by several spectroscopic techniques in phosphate buffer aqueous solutions. UV−vis, circular dichroism, and fluorescence spectroscopic data confirm that intercalation is the prevailing mode of interaction, and also reveal that the interaction with AT-rich regions leads to the transfer of excitation energy to DX not previously documented in the literature. Moreover, the DX affinity for AT sites has been found to be on the same order of magnitude as that reported for GC sites.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2014-01-01 |