6533b825fe1ef96bd1282695
RESEARCH PRODUCT
A rapid spectrofluorimetric method for the determination of the degree of synchronism inSaccharomyces cerevisiae
Rafael SentandreuSalvador Mormeneosubject
ChromatographybiologySaccharomyces cerevisiaeFluorescence spectrometryGeneral Medicinebiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyFluorescencechemistry.chemical_compoundBiochemistrychemistrySynchronismDNAdescription
A fluorescence technique that allows direct measurement of DNA and synchrony levels inSaccharomyces cerevisiae cell cultures is reported. The spectrofluorimetric estimation of DNA with 4,6-diamidino-2-phenylindole (AT-dye) does not require prior extraction, is highly stable, and requires small quantities of cells.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1985-07-01 | Current Microbiology |