6533b825fe1ef96bd1282a1b
RESEARCH PRODUCT
Voltage gradient electrophoresis of nucleic acids on agarose gels.
Giovanni DuroGiovanni GiudiceMaria Assunta CostaRainer BarbieriVincenzo Izzosubject
Gel electrophoresisElectrophoresis Agar GelChromatographyGel electrophoresis of nucleic acidsChemistryBiophysicsCell BiologyDNABiochemistryBuffer (optical fiber)Molecular WeightElectrophoresischemistry.chemical_compoundEvaluation Studies as TopicAgarose gel electrophoresisPulsed-field gel electrophoresisNucleic acidAgaroseMolecular BiologyPlasmidsdescription
A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1993-07-01 | Analytical biochemistry |