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RESEARCH PRODUCT
Immunoanalytical methods for ochratoxin A monitoring in wine and must based on innovative immunoreagents.
Antonio Abad-fuentesJosep V. MercaderDaniel López-puertollanoConsuelo AgullóAntonio Abad-somovillasubject
Ochratoxin AMonoclonal antibodyFood ContaminationWine01 natural sciencesAnalytical ChemistryFood safetychemistry.chemical_compound0404 agricultural biotechnologyLimit of DetectionScreening methodDipstickMycotoxinWineImmunoassayMycotoxinChromatographybusiness.industry010401 analytical chemistryfood and beverages04 agricultural and veterinary sciencesGeneral MedicineDipstickContaminationFood safety040401 food scienceOchratoxins0104 chemical sciencesHaptenchemistryCompetitive immunoassayCompetitive immunoassayEnvironmental scienceIndicators and ReagentsbusinessFood AnalysisFood Sciencedescription
Immunochemical methods are highly deployed in analytical laboratories worldwide for monitoring the incidence of mycotoxins in the food chain. Nevertheless, most conventional immunoassays for ochratoxin A (OTA), including commercial kits, show limitations to robustly determine this mycotoxin in grape-derived products below regulated levels (2 ng/mL). Herein, two rapid tests for sensitive OTA determination in wine and must were developed capitalizing on a collection of bioconjugates from innovative synthetic haptens and monoclonal antibodies with subnanomolar affinity. The ELISA (LOD = 8 pg/mL) showed excellent performance in recovery studies, and it was applied to survey commercial wines and musts for OTA contamination. Concerning LFIA, validation according to the Commission Regulation 519/2014 showed that samples exceeding 2 ng/mL were properly scored as uncompliant. More importantly, illegal samples provided a complete inhibition of the test signal, making this test an easy-to-use, rapid, and convenient screening method for in-house control of OTA in wineries.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2020-12-08 | Food chemistry |