Maizes rauga adenīna biosintēzes ceļa mutāciju ietekme uz oksidatīvā un karstuma stresu izturību.

6533b825fe1ef96bd1282cf1

RESEARCH PRODUCT

Maizes rauga adenīna biosintēzes ceļa mutāciju ietekme uz oksidatīvā un karstuma stresu izturību.

Sandra Seimuškina

subject

maizes raugs glikozes 6-fosfāta dehidrogenāze karstuma oksidatīvā stresa izturība kalatāze Bioloģija

description

Maizes raugs ir nozīmīgs modeļorganisms, lai praktiski pētītu adenīna biosintēzes ceļa mutāciju ietekmi uz oksidatīvā un karstuma stresu izturību, nepievienojot tiem barības vielas. Bakalaura darba ietvaros pētīta adenīna biosintēzes ceļa mutantu ietekme uz oksidatīvo un karstuma stresu, katalāzes un glikozes 6-fosfāta dehidrogenāzes enzīmu aktivitāte. Vispirms atrasti optimālie ilgumi šūnu pakļaušanai stresiem, tad noskaidrots, ka purīna un slāpekļa badināšana palielina stresa izturību neatkarīgi no mutācijas vietas adenīna sintēzes ceļā. Iegūtie rezultāti norāda, ka katalāze uzrāda augstāku aktivitāti pie augstākas oksidatīvā stresa izdzīvotības W303 un Δade, Δade2 celmiem, bet ne Δade8, savukārt glikozes 6-fosfāta dehidrogenāzes aktivitāte nekorelē ar oksidatīvā stresa izturību. Visiem adenīna biosintēzes mutāciju celmiem netika atrastas kopīgas tendences katalāzes unz glikozes 6-fosfāta dehidrogenāzes mērījumos.

year	journal	country	edition	language
2021-01-01

https://dspace.lu.lv/dspace/handle/7/54467

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