6533b825fe1ef96bd128318d

RESEARCH PRODUCT

Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5

Mika J. WallénMikko O. LaukkanenMarkku S. Kulomaa

subject

CloningGeneticsNucleic acid sequenceIntronGeneral MedicineBiologyMolecular biologyExonComplementary DNAGeneticsbiology.proteinCoding regionGeneAvidin

description

Abstract The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91–95%) with the previously isolated Avd-related genes 1–5 (Avrl–Avr5) . Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.

yearjournalcountryeditionlanguage
1995-08-01Gene
https://doi.org/10.1016/0378-1119(95)00187-b