6533b825fe1ef96bd12832be
RESEARCH PRODUCT
Isolation and partial characterization of uronic acid-containing glycoproteins from Mucor rouxii.
Arturo Flores-carreónArturo Flores-carreónRoberto Zazueta-sandovalSalvador Mormeneosubject
ChromatographyMolecular massIon exchangeFraction (chemistry)General MedicineUronic acidGlucuronic acidApplied Microbiology and BiotechnologyMicrobiologyCell wallFungal Proteinschemistry.chemical_compoundUronic AcidschemistryBiochemistryCytoplasmMucorSodium dodecyl sulfatedescription
Five different fractions containing uronic acids associated with protein were isolated from the cytoplasm of the filamentous form of Mucor rouxii. A signle fraction was isolated from the cell wall by hot sodium dodecyl sulfate followed by ion exchange column chromatography. Two cytoplasmic entities (peaks I and II) were not adsorbed to DEAE Bio-Gel A. The molecular mass of peaks I to V ranged from 16.5 to 210 kDa. The protein-uronic acid ratios were different for each fraction. The cell wall fraction showed a molecular mass of 16.5 kDa, similar to that of peak II but with differences in chromatographic behavior and protein-uronic acid ratio. The possible role of these molecules as acceptors of sugar residues during polyuronide chain growth is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1995-04-01 | Current microbiology |