6533b826fe1ef96bd1283c7c
RESEARCH PRODUCT
Influence of culture conditions on embryo formation and maturation in auxin-induced embryogenic cultures of Digitalis obscura.
M. C. BrisaLeonor Lapeñasubject
chemistry.chemical_classificationSomatic embryogenesisDigitalis obscuraEmbryoPlant ScienceGeneral MedicineBiologybiology.organism_classificationchemistry.chemical_compoundchemistryBiochemistryAuxinCell cultureSubculture (biology)Agronomy and Crop ScienceAbscisic acidGibberellic aciddescription
Somatic embryogenesis was induced in hypocotyls of Digitalis obscura using indoleacetic acid or 2,4-dichlorophenoxyacetic acid with different culture and subculture conditions. Indoleacetic acid-induced embryogenic cultures were used to investigate the effects of amino acids, polyamines and growth regulators on embryo differentiation and maturation. Supplementation of the media with amino acids, polyamines or abscisic acid did not influence or had an adverse effect on embryogenic response. Gibberellic acid at 1.4 μM in either culture (30 days) or subculture medium was effective in promoting both differentiation and normal embryo development. The efficiency of somatic embryogenesis was greatly enhanced when isolated indoleacetic acid-induced proembryogenic masses were subcultured in liquid medium with reduced auxin content.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-02-07 | Plant cell reports |