6533b826fe1ef96bd1284551
RESEARCH PRODUCT
[35] Determination of oxidized glutathione in blood: High-performance liquid chromatography
Juan SastreJose ViñaJosé M. EstrelaMiguel AsensiFederico V. Pallardósubject
inorganic chemicalsHuman studiesbiologyChemistryGlutathionemedicine.disease_causeHigh-performance liquid chromatographyEnzyme assayOxidized Glutathionechemistry.chemical_compoundfluids and secretionsBiochemistrymedicinebiology.proteinChelationIntracellularOxidative stressdescription
Publisher Summary The measurement of glutathione status is important in determining oxidative stress in tissues and biological fluids. The ratio of reduced to oxidized glutathione (GSH/GSSG) is a good indicator of the oxidative stress that may occur under physiological and pathological conditions. Changes in GSSG levels have been considered as intracellular signals able to modulate enzyme activity. Thus, it is important to have accurate methods to determine GSSG in biological fluids and in cells. In many cases, it is possible to use tissues, such as liver, muscle, or brain to determine GSH/GSSG. However, especially in human studies, samples from these tissues are not readily available; the measurement of blood samples is required. A major problem is the measurement of GSSG in the presence of GSH because spontaneous or catalyzed GSH oxidation must be prevented. Several methods have been developed to measure GSH and GSSG. Most methods rely on chelating thiols to prevent GSH oxidation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1994-01-01 |