6533b826fe1ef96bd12846bb

RESEARCH PRODUCT

Candida albicans ABG1 gene is involved in endocytosis.

Manuel CasanovaAmelia MurguiJosé P. MartínezVeronica VesesNeil A. R. Gow

subject

Hyphal growthFungal proteinRecombinant Fusion ProteinsfungiSpitzenkörperGreen Fluorescent ProteinsHyphaeGerm tubeGeneral MedicineVacuoleBiologybiology.organism_classificationEndocytosisApplied Microbiology and BiotechnologyMicrobiologyEndocytosisCell biologyArtificial Gene FusionFungal ProteinsEndocytic vesicleMicroscopy FluorescenceGenes ReporterCandida albicansHumansCandida albicans

description

The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in endocytosis.

yearjournalcountryeditionlanguage
2009-03-01FEMS yeast research
10.1111/j.1567-1364.2009.00480.xhttps://pubmed.ncbi.nlm.nih.gov/19175413