6533b826fe1ef96bd12846e9
RESEARCH PRODUCT
Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs
Johan E. PeetersIlka B. EeckhoutHans De ConinckDirk C. De GraafFranz Petrysubject
Antigenicityanimal diseasesMolecular Sequence DataProtozoan ProteinsAntibodies ProtozoanAntigens ProtozoanMolecular cloningBrief Communicationlaw.inventionAntigenlawparasitic diseasesAnimalsAmino Acid SequenceZinc fingerCryptosporidium parvumExpression vectorbiologyBase SequencecDNA libraryZinc Fingersbiology.organism_classificationVirologyRecombinant ProteinsInfectious DiseasesCryptosporidium parvumRecombinant DNAParasitologyCattleRabbitsdescription
A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-03-01 |