6533b826fe1ef96bd1284762
RESEARCH PRODUCT
Expression and distribution of the glucocorticoid receptor DlGR1 in the teleost Dicentrarchus labrax brain
Maria Luigia Di BellaGiuseppina SalernoAiti VizziniMonica CeliNicolò ParrinelloMirella Vazzanasubject
Messenger RNAD. labraxSettore BIO/05 - ZoologiaRiboprobeIn situ hybridizationBiologyD. labrax Immunohistochemistry In situ hybridization Glucocorticoid receptorMolecular biologyGlucocorticoid receptorHormone receptorComplementary DNAimmunohistochemistryglucocorticoid receptorglucocorticoidAnimal Science and Zoologyin situ hybridizationlcsh:Animal cultureReceptorTranscription factorlcsh:SF1-1100description
Cortisol is the main corticosteroid secreted by the interrenal cells of the head kidney and it exerts a role in mantaining the omeostatic status in fish. In teleosts its effects are mediated through intracellular receptors expressed in several tissues, that are ligand-dependent transcription factors by binding to specific tissue DNA sequences. In Dicentrarchus labrax we previously cloned and sequenced a glucocorticoid receptor, DlGR1, isolated from leukocytes of peritoneal cavity. In this work we showed mRNA expression and tissue immunohistochemical localization of brain DlGR1 by in situ hybridization assays, with a riboprobe with DlGR1 cDNA trascriptional activation domain, and by immunohistochemical methods, using a specific antibody for a selected sequence of the receptor tran- scriptional domain. The mRNA and the protein are expressed in pyramidal cells of the optic lobe and in the small globular neurons of the diencephalon.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2009-01-01 |