6533b826fe1ef96bd12848dd

RESEARCH PRODUCT

Activation of a murine autoreactive B cell by immunization with human recombinant autoantigen La/SS-B: Characterization of the autoepitope

G. PollakG. PollakW.j. Van VenrooijW.j. Van VenrooijH. BartschH. BartschG.j.m. PruijnG.j.m. PruijnHelmut TrösterMichael BachmannMichael BachmannImre SemseiImre SemseiMartin SchwemmleMartin SchwemmleT.e. MetzgerT.e. MetzgerR. KleinR. Klein

subject

medicine.drug_classRecombinant Fusion ProteinsImmunologyMolecular Sequence DataAutoimmunityBiologyMonoclonal antibodyAutoantigensPC12 CellsEpitopelaw.inventionCell LineMiceAdenosine TriphosphatelawmedicineImmunology and AllergyAnimalsHumansElméleti orvostudományokAmino Acid SequenceGeneralLiterature_REFERENCE(e.g.dictionariesencyclopediasglossaries)Peptide sequencechemistry.chemical_classificationB-LymphocytesMicroscopy ConfocalLinear epitopeOrvostudományok3T3 CellsMolecular biologyAmino acidRatschemistryMicroscopy FluorescenceRibonucleoproteinsRecombinant DNAbiology.proteinCattleImmunizationAntibodyEpitope MappingGene DeletionConformational epitope

description

Immunization of Balb/c mice with a homogeneously purified recombinant human La/SS-B protein resulted in activation of an autoreactive B cell secreting a novel monoclonal anti-La antibody termed La4B6. La4B6 reacted with La protein from a variety of sources including human, bovine, rat and mouse. ATP blocked the binding of La4B6 to recombinant La protein. The human epitope was identified as consisting of the amino acid sequence SKGRRFKGKGKGN, which includes the proposed ATP-binding site of the La protein. In the human and bovine La protein, the epitope exists as a continuous amino acid sequence. In rat and mouse the epitope was found to consist of the amino acid sequence SKG interrupted by a species-specific insert of 16 amino acids, and followed by the second half of the epitope, the amino acid sequence RRFKGKGKGN. Our data suggest that in the case of the rat and mouse La proteins the two separated parts of the epitope are able to form a conformational epitope which looks similar to the continuous human epitope.

yearjournalcountryeditionlanguage
1995-12-01
https://hdl.handle.net/2066/28364