6533b826fe1ef96bd12848ed
RESEARCH PRODUCT
Cercospora beticola toxins. Use of fluorescent cyanine dye to study their effects on tobacco cell suspensions
Jean-pierre BleinMarie-louise MilatVladimír MikešAlain PuginFrançoise Simon-plasIsabelle Gapilloutsubject
0106 biological sciencesCyanideATPasePlant ScienceHorticultureBiology01 natural sciencesBiochemistry03 medical and health scienceschemistry.chemical_compoundCyanineMolecular Biology[SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyComputingMilieux_MISCELLANEOUS030304 developmental biologyMembrane potential0303 health sciencesGeneral MedicineCercospora beticolabiology.organism_classificationFluorescence[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyMembranechemistryBiochemistrybiology.proteinAzide010606 plant biology & botanydescription
Abstract The fluorescent dye 3,3′-diethylthiadicarbocyanine iodide [diS-C 2 -(5)] was used to observe plasmalemma transmembrane potential variations of tobacco cells treated with uncoupler (FCCP), respiratory inhibitors (azide and cyanide), and H + -ATPase inhibitors (DCCD and a carbanilate derivative). These chemicals induced an increase in fluorescence, indicating a dissipation of the transmembrane potential. The [diS-C 2 -(5)] was also used to study the effects of two Cercospora beticola toxins on tobacco cells. Changes in fluorescence of [diS-C 2 -(5)] suggested that these two toxins caused a dissipation of the transmembrane potential with a different magnitude whereas kinetics of their association with membranes were comparable.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-09-01 |