6533b826fe1ef96bd128491e
RESEARCH PRODUCT
Expression of R-3-hydroxybutyrate dehydrogenase, a ketone body converting enzyme in heart and liver mitochondria of ruminant and non-ruminant mammals
Arlette KanteNorbert LatruffeMustapha Cherkaoui MalkiChristian Demignésubject
medicine.medical_specialtyPhysiologyBlotting WesternMitochondria LiverDehydrogenaseCross ReactionsBiologyMitochondrionKidneyBiochemistryMitochondria HeartHydroxybutyrate DehydrogenaseInternal medicinemedicineAnimalsHumansMolecular Biologychemistry.chemical_classificationKidneySheepGeneral MedicineEnzyme assayRatsCytosolEnzymemedicine.anatomical_structureEndocrinologyLiverchemistryBiochemistryMicrosomeKetone bodiesbiology.proteinCattledescription
1. The properties of rat liver and bovine heart R-3-hydroxybutyrate dehydrogenase (BDH) have been extensively studied in the past 20 years, but little is known concerning the biogenesis and the regulation of this dehydrogenase over different species. 2. In addition, controversial results were often reported concerning the activity, the level and the subcellular location of this enzyme in ruminants. 3. BDH activity found in liver and kidney mitochondria from ruminants (cow and sheep) is low, while it is much higher in rat. 4. However, the enzyme activity is detected in microsomes and in cytosol of liver and of kidney cells from ruminants. These activities are not correlated to ketonaemia level. 5. Although low BDH activity is detected in liver mitochondria from ruminants; the bovine liver BDH gene seems to be translated since BDH can be immunodetected by using an antiserum raised against bovine heart BDH. 6. Beside this, the good cross-reactivity between heart BDH and liver BDH suggests their high level of homology in ruminants.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-03-01 | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry |