Kolistīna rezistento Escherichia coli izolātu ģenētiskais raksturojums

6533b826fe1ef96bd1284b2b

RESEARCH PRODUCT

Kolistīna rezistento Escherichia coli izolātu ģenētiskais raksturojums

subject

Escherichia coli kolistīna Bioloģija rezistence mcr-1

description

Antibiotiku rezistence ir aktuāla problēma, kas izraisījusi satraukumu par iespējām ārstēt bakteriālās infekcijas. Kolistīns ir plaša spektra antibiotika, kas tiek lietota pret multirezistentiem baktēriju celmiem. Rezistenci pret kolistīnu nodrošina mcr gēni, kas var būt kodēti plazmīdās un hromosomās. Pētījumā noteikta kolistīna rezistence pārtikas un cūku aklo zarnu paraugos. Paraugi iegūti Valsts Pētījumu Programmas “Lauksaimniecības resursi ilgtspējīgai kvalitatīvas un veselīgas pārtikas ražošanai Latvijā (AgroBioRes)” projekta Nr. 5 “Mikroorganismu rezistences un citu bioloģisko un ķīmisko risku izpētes procedūru izstrāde un pielietošanas pārtikas ķēdē (RISKI)” ietvaros no 2015. līdz 2018.gadam. Kopā tika raksturoti 354 Escherichia coli izolāti. Ar minimālās inhibējošās koncentrācijas metodi kolistīna rezistence noteikta 13 paraugos, savukārt mcr-1 gēna klātbūtne konstatēta 16 paraugos. Veicot nākošās paaudzes sekvenēšanu (NGS) noskaidrots, ka visos mcr-1 gēna saturošajos izolātos atrodas vienādi insercijas sekvenču elementi un IncX4 plazmīda, kas, iespējams, saistīta ar kolistīna rezistences pārnesi starp baktērijām. Ar pulsējošā lauka gela elektroforēzi un NGS datiem noskaidrota pārtikas izolātu ģenētiskā radniecība ar cūku aklo zarnu izolātiem.

year	journal	country	edition	language
2018-01-01

https://dspace.lu.lv/dspace/handle/7/39643

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