6533b827fe1ef96bd128542f

RESEARCH PRODUCT

Soybean Diseases Caused by Genus Pseudomonas Phytopathenes Bacteria

subject

description

The rationale and experimental confirmation of the role of the bacteria of the Pseudomonas genus in the pathogenesis of soybean, and the investigation of the biological properties of the main causative agent Pseudomonas savastanoi pv. glycines. Methods. Theoretical generalization, laboratory-model research, phytopathological, microbiological, physiological, biochemical and genetic methods. Results. The largest group of pathogens in different soil-climatic zones of Ukraine consists of bacteria of the genus Pseudomonas was established. Their biological properties have been investigated and the methods for identification of the dominant type of phytopathogenic bacteria causing bacterial blight of soybean, Pseudomonas savastanoi pv. glycinea, have been proposed. Conclusions. Long-term soybean crops monitoring of regionalized varieties in different soil-climatic zones of Ukraine has showm that the one of the dominant bacterial pathogens is Pseudomonas savastanoi pv. glycinea in the range of bacterial pathogens of this plant. A database of BOX-fragments profiles for P. savastanoi pv. glycinea, P. syringae pv. syringae, P. savastanoi pv. phaseolicola, Pseudomonas syringae pv. tabaci collections strains was created. The BOX-PCR method allows identification phytopathogenic pseudomonads to the species level, but not to the pathovar. Was shown that for Pseudomonas soybean bacteriosis identification should be used symptomatology and polyphase taxonomy methods