6533b827fe1ef96bd1285b4e

RESEARCH PRODUCT

On the role of D-glucuronic acid in the aggregation of cells from the marine sponge Geodia cydonium.

Werner E.g. MüllerGerd UhlenbruckP. Vaith

subject

Binding SitesbiologyImmunologyGalactoseGlucuronatesCarbohydratebiology.organism_classificationGlucuronic acidBinding CompetitiveGalactosidasesPoriferaSpongechemistry.chemical_compoundAgglutininGlucosechemistryBiochemistryConcanavalin ALimulusGalactosebiology.proteinAnimalsGeodiaDevelopmental BiologyCell Aggregation

description

Abstract The aggregation receptor (AR) from the marine sponge GEODIA CYDONIUM was analyzed with respect to its monosaccharide composition. Three major sugars ( D -galactose, D -glucose and D -glucuronic acid) accounted for about 85 % of the total carbohydrate. Negative results with different lectins directed against D -galactosyl, N -acetyl- D -galactosaminyl and N -acetyl- D -glucosaminyl groups, respectively, showed that these sugars are serologically unreactive in AR. Positive serological reactions were obtained with CONCANAVALIN A and LIMULUS POLYPHEMUS agglutinin. AR also reacted strongly with the basic polymer poly- L -lysine. Reaggregation experiments performed on the basis of these findings strongly suggest that AR-bound D -glucuronic acid is recognized by an intercellular aggregation factor (AF) during the secondary aggregation of GEODIA cells.

10.1016/s0145-305x(79)80023-3https://pubmed.ncbi.nlm.nih.gov/478075