6533b827fe1ef96bd1285cf5
RESEARCH PRODUCT
Extracellular Vesicles From Liver Progenitor Cells Downregulates Fibroblast Metabolic Activity and Increase the Expression of Immune-Response Related Molecules
Felix RoyoFelix RoyoMikel AzkargortaJose L. LavinMarc Clos-garciaAna R. CortazarAna R. CortazarMonika Gonzalez-lopezLaura BarcenaHernando A. Del PortilloHernando A. Del PortilloHernando A. Del PortilloMaría Yáñez-móAntonio MarcillaAntonio MarcillaFrancesc E. BorràsFrancesc E. BorrasFrancesc E. BorrasHector PeinadoIsabel GuerreroMar Váles-gómezUnai CereijoTeresa SardonAna M. AransayAna M. AransayFelix ElortzaFelix ElortzaJuan M. Falcon-perezJuan M. Falcon-perezJuan M. Falcon-perezsubject
MLP29ChemistryMLP29 cell crosstalk exosomes extracellular vesicles (EVs) fibroblast immune responseCell BiologyexosomesCell fate determinationcell crosstalkMicrovesiclesfibroblastimmune responseCell biologyCell and Developmental BiologyCXCL2Crosstalk (biology)Immune systemmedicine.anatomical_structurelcsh:Biology (General)Downregulation and upregulationmedicineProgenitor cellextracellular vesicles (EVs)Fibroblastlcsh:QH301-705.5Original ResearchDevelopmental Biologydescription
Extracellular vesicles (EVs) mediate cell-to-cell crosstalk whose content can induce changes in acceptor cells and their microenvironment. MLP29 cells are mouse liver progenitor cells that release EVs loaded with signaling cues that could affect cell fate. In the current work, we incubated 3T3-L1 mouse fibroblasts with MLP29-derived EVs, and then analyzed changes by proteomics and transcriptomics. Results showed a general downregulation of protein and transcript expression related to proliferative and metabolic routes dependent on TGF-beta. We also observed an increase in the ERBB2 interacting protein (ERBIN) and Cxcl2, together with an induction of ribosome biogenesis and interferon-related response molecules, suggesting the activation of immune system signaling.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-01-12 |