6533b828fe1ef96bd1287789

RESEARCH PRODUCT

Relationships between oxygen concentration and patterns of energy metabolism in the rotifer Brachionus plicatilis

Manuel SerraM. Rosa MiracleAngeles Esparcia

subject

AlaninebiologyPhysiologyMalic enzymeGeneral MedicineBrachionusbiology.organism_classificationBiochemistryMalate dehydrogenasechemistry.chemical_compoundBiochemistrychemistryLactate dehydrogenasebiology.proteinLimiting oxygen concentrationMolecular BiologyPyruvate kinaseAlcohol dehydrogenase

description

Abstract 1. 1. Activities of 10 enzymes and accumulation of two metabolites under experimental hypoxia were investigated in the rotifer Brachionus plicatilis . 2. 2. Pyruvate kinase (PK), lactate dehydrogenase (LDH), malic enzyme (ME), phosphoenolpyruvate carboxykinase (PEPCK), malate dehydrogenase (MDH) and fumarase (FUM) activities showed great increase at low oxygen levels. Alcohol dehydrogenase (ADH) activity was not detected. 3. 3. Alanine concentration showed an increase with low oxygen concentrations, while alanine aminotransferase (GTP) showed a slight activity increase with oxygen levels. The high alanine concentration was interpreted as enhanced proteolysis. 4. 4. Our results showed that the rotifers used the lactate pathway for anoxic glucose catabolism. Data also suggested that the glucose-succinate pathway would function at low oxygen concentrations. This pattern is consistent with the phylogenetic position of rotifers.

yearjournalcountryeditionlanguage
1992-10-01Comparative Biochemistry and Physiology Part B: Comparative Biochemistry
https://doi.org/10.1016/0305-0491(92)90304-a