6533b828fe1ef96bd12882d9
RESEARCH PRODUCT
Separation and Biochemical Characterization of Rat Liver Parenchymal Cell Subpopulations
Beate SeibertFranz OeschPablo Steinbergsubject
CytochromebiologyChemistryRat liverParenchymamedicinebiology.proteinHepatotoxinPhenobarbitalLobules of liverMonooxygenaseMolecular biologymedicine.drugdescription
Parenchymal cells within the hepatic lobules of the rat are morphologically and biochemically heterogeneous (Shank et al., 1959; Novikoff, 1959; Loud, 1968; Jungermann and Katz, 1982). It has been previously shown that the concentration of cytochrome P-450 and the activities of most cytochrome P-450-dependent monooxygenases are relatively higher in the centrilobular regions than in the periportal regions of the rat liver (Baron et al., 1978; Gooding et al., 1978; Baron and Kawabata, 1983); further, these studies revealed that pretreatment of the animals with phenobarbital intensified this gradation across the liver lobule, whereas after administration of 3-methylcholanthrene the concentration of cytochrome P-450 and the monooxygenase activities were more uniformly distributed. Since very reactive and toxic metabolites are often formed during cytochrome P-450-catalyzed monooxygenations of hepatotoxins, the intralobular distribution of different cytochromes P-450 may be a crucial factor in determining the location of the damage within the hepatic lobules after exposure to several hepatotoxic compounds.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-01-01 |