6533b828fe1ef96bd128834a
RESEARCH PRODUCT
Studies on sea urchin oocytes. II. Synthesis of RNA during oogenesis.
Ida AlbaneseAntonio BonoGabriella SconzoGiovanni Giudicesubject
SucroseTime FactorsBiologyCell FractionationTritiumOogenesisParacentrotus lividusbiology.animalBotanyCentrifugation Density GradientAnimalsPolyacrylamide gel electrophoresisSea urchinUridineOvumCell NucleusHistocytochemistryEmbryogenesisRNACell BiologyRibosomal RNAbiology.organism_classificationElectrophoresis DiscMolecular WeightBiochemistryRNA RibosomalSea UrchinsAutoradiographyRNAFemaleCell fractionationCell Nucleolusdescription
Abstract Isolated oocytes of the sea urchin Paracentrotus lividus actively incorporate 3H-uridine into RNA. Labeled RNA was analysed by sucrose gradient and acrylamide gel electrophoresis following cell fractionation. Much of the radioactivity is incorporated at the nucleolar level in the form of rRNA precursors. The kinetics of maturation of these latter suggests that this occurs at a slower rate than during embryogenesis. Other non-nucleolar RNA classes are also actively labelled and retained in the nucleus for many hours. These results are confirmed by an autoradiographic investigation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1972-05-01 | Experimental cell research |